Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Capillary electrophoresis system for hemoglobin A1c determinations evaluated

C J Doelman1, C W Siebelder, W A Nijhof

  • 1Department of Clinical Chemistry and Hematology, Queen Beatrix Hospital, Winterswijk, The Netherlands.

Clinical Chemistry
|April 1, 1997
PubMed
Summary

Capillary electrophoresis offers a new method for measuring Hemoglobin A1c (Hb A1c), crucial for diabetes management. This technique accurately determines Hb A1c levels, unaffected by common interfering substances or hemoglobin variants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Management of high-grade neuroendocrine neoplasms: impact of functional imaging.

Endocrine-related cancer·2025
Same author

Efficacy of inflammation-based stratification for add-on celecoxib or minocycline in major depressive disorder: Protocol of the INSTA-MD double-blind placebo-controlled randomised clinical trial.

Brain, behavior, & immunity - health·2024
Same author

Non-esophageal eosinophilic gastrointestinal diseases: a narrative review.

Acta gastro-enterologica Belgica·2023
Same author

A single-centre analysis of post-colonoscopy colorectal cancer.

Acta gastro-enterologica Belgica·2021
Same author

Current practice in approaching controversial diagnostic and therapeutic topics in gastroenteropancreatic neuroendocrine neoplasm management. Belgian multidisciplinary expert discussion based on a modified Delphi method.

Acta gastro-enterologica Belgica·2020
Same author

Development of Precision Small-Molecule Proneurotrophic Therapies for Neurodegenerative Diseases.

Vitamins and hormones·2017

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Hemoglobin A1c (Hb A1c) is the standard analyte for assessing long-term glycemic control in diabetes mellitus patients.
  • Accurate Hb A1c measurement is vital for effective diabetes management and preventing complications.
  • Existing methods may be subject to interference from various factors, impacting reliability.

Purpose of the Study:

  • To introduce and evaluate capillary electrophoresis as a novel analytical technique for Hb A1c determination.
  • To assess the specificity and accuracy of capillary electrophoresis for Hb A1c measurement.
  • To determine if common interfering substances and hemoglobin variants affect the capillary electrophoresis method.

Main Methods:

  • Capillary electrophoresis was employed as the primary analytical technique for Hb A1c measurement.

Related Experiment Videos

  • The method's performance was evaluated by assessing its independence from labile Hb A1c fractions.
  • Interference from carbamylated hemoglobin, acetylated hemoglobin, and common hemoglobin variants (Hb F, Hb S, Hb C) was investigated.

    • Main Results:

    • The capillary electrophoresis method provides accurate Hb A1c determination.
    • The measurement is not influenced by the labile Hb A1c fraction.
    • Carbamylated hemoglobin, acetylated hemoglobin, and hemoglobin variants (Hb F, Hb S, Hb C) do not interfere with the analysis.

    Conclusions:

    • Capillary electrophoresis is a reliable and valuable analytical tool for clinical laboratories.
    • This technique offers an accurate method for measuring Hb A1c, essential for diabetes monitoring.
    • The method's robustness against common interferences enhances its clinical utility.