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Updated: Aug 7, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Semiquantitative RT-PCR: enhancement of assay accuracy and reproducibility

T Sarić1, S A Shain

  • 1Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio 78284-7836, USA.

Biotechniques
|April 1, 1997
PubMed
Abstract

No abstract available in PubMed .

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PCR01:32

PCR

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...