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Liquid chromatographic measurement of L-ascorbic acid and D-ascorbic acid in biological samples
1Chemical Standards and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Abstract:
D- and L-Ascorbic acids have been separated using liquid chromatography (LC) on a polymer-coated silica-based NH2 column and the L-isomer has been quantified in human serum, rat serum, rat lung, rat lung perfusate, infant formula (SRM 1846) and mixed food sample (SRM 2383). The D-isomer was observed only in trace amounts in the mixed food sample. The results demonstrate that ascorbic acid was stable on the column and completely recovered from supplemented samples of human serum and that this method of analysis is accurate, precise and has broad application exhibiting no dependence on the nature of the matrices evaluated herein.