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High-performance liquid chromatographic assay for cefepime in serum
H Elkhaïli1, L Linger, H Monteil
1Laboratory of Pharmacokinetics, University Hospital Center, Strasbourg, France.
Summary
A new high-performance liquid chromatography method accurately quantifies cefepime in human serum. Cefepime is unstable at room temperature and requires storage at -80°C for delayed analysis.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Cefepime is a fourth-generation cephalosporin antibiotic with broad-spectrum activity.
- Accurate quantification of cefepime in biological matrices is crucial for therapeutic drug monitoring and pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a simple, rapid, specific, and sensitive high-performance liquid chromatographic (HPLC) method for determining cefepime in human serum.
Main Methods:
- HPLC separation was performed on a reversed-phase Ultrasphere XL-ODS column.
- The mobile phase consisted of 7% acetonitrile in 20 mM ammonium acetate (pH 4).
- Detection was achieved using UV absorbance at 254 nm.
Main Results:
- The method demonstrated excellent linearity (R² > 0.999) over a range of 0.1 to 50 µg/mL.
- The lower limit of quantitation was 0.5 µg/mL with high recovery (106.2 ± 2.1%).
- Cefepime exhibited instability in serum at room temperature, necessitating storage at -80°C.
Conclusions:
- The developed HPLC method is suitable for the routine determination of cefepime in human serum.
- Proper sample storage at -80°C is essential to maintain cefepime stability for accurate analysis.