Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytokine-mediated neuronal apoptosis

S Hu1, P K Peterson, C C Chao

  • 1Neuroimmunobiology and Host Defense Laboratory, Minneapolis Medical Research Foundation, MN 55404, USA.

Neurochemistry International
|April 1, 1997
PubMed
Summary

Cytokines trigger neuronal death through nitric oxide (NO) in human neurons. Inhibiting NO prevents this cell death, suggesting new therapies for neurodegenerative diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of contrast-enhanced ultrasonography and MRI results obtained by expert and novice radiologists indicating short-term response after transarterial chemoembolisation for hepatocellular carcinoma.

Clinical radiology·2023
Same author

Serological evidence of high Leptospira exposure among indigenous people (Orang Asli) in Peninsular Malaysia using a recombinant antigen-based ELISA.

Tropical biomedicine·2021
Same author

Role of Iron in the Cellular Effects of Asbestos.

Inhalation toxicology·2015
Same author

Psychological linguistics: A natural science approach to the study of language interactions.

The Analysis of verbal behavior·2012
Same author

Selective drug efflux in multidrug-resistant immunoblastic B lymphoma cells with overexpressed P-glycoprotein.

Environmental toxicology and pharmacology·2011
Same author

Inhibition by arsenite of anticancer drug cis-diamminedichloroplatinum(II) induced DNA repair and drug resistance in HeLa cells.

Environmental toxicology and pharmacology·2011

Area of Science:

  • Neuroscience
  • Immunology

Background:

  • Cytokines are implicated in neuronal injury via nitric oxide (NO), but the exact mechanism remains unclear.
  • Understanding cytokine-mediated neurotoxicity is crucial for developing treatments for neurodegenerative conditions.

Purpose of the Study:

  • To investigate if cytokine-induced neurotoxicity in human neurons involves apoptosis triggered by NO.
  • To explore the therapeutic potential of inhibiting NO in cytokine-mediated neurotoxicity.

Main Methods:

  • Primary human fetal neurons and glial cells were treated with interferon-gamma (IFN-γ) and interleukin-1 beta (IL-1β).
  • Nitric oxide synthase inhibitor N-monomethyl-L-arginine (NMMA) was used to block NO production.
  • Neuronal loss, NO output, and DNA fragmentation were assessed.

Main Results:

  • Cytokine treatment led to high NO output and significant neuronal loss.
  • NMMA treatment attenuated cytokine-induced neuronal loss, confirming NO's role.
  • Neuronal injury exhibited apoptotic features, including morphological changes and DNA fragmentation.

Conclusions:

  • Cytokine-mediated neurotoxicity in human neurons proceeds via an NO-triggered apoptotic mechanism.
  • Inhibiting NO offers a potential therapeutic strategy for neurodegenerative diseases involving glia, cytokines, and NO.

Related Experiment Videos