Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cardiolipin-dependent decrease of cytochrome c oxidase activity in heart mitochondria from hypothyroid rats

G Paradies1, G Petrosillo, F M Ruggiero

  • 1Department of Biochemistry and Molecular Biology, University of Bari, Italy.

Biochimica Et Biophysica Acta
|March 28, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cigarette toxicity triggers Leber's hereditary optic neuropathy by affecting mtDNA copy number, oxidative phosphorylation and ROS detoxification pathways.

Cell death & disease·2015
Same author

A cross-sectional nationwide survey on esophageal atresia and tracheoesophageal fistula.

Journal of pediatric surgery·2015
Same author

VATS: first step in the parapneumonic empyema*.

Il Giornale di chirurgia·2014
Same author

[Gartner's duct cyst: report of three cases].

La Pediatria medica e chirurgica : Medical and surgical pediatrics·2012
Same author

In vivo hyperoxic preconditioning protects against rat-heart ischemia/reperfusion injury by inhibiting mitochondrial permeability transition pore opening and cytochrome c release.

Free radical biology & medicine·2010
Same author

Mitochondrial dysfunction in rat brain with aging Involvement of complex I, reactive oxygen species and cardiolipin.

Neurochemistry international·2008
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Thyroid hormone deficiency reduces heart mitochondrial cytochrome oxidase activity. Supplementing with cardiolipin (a key mitochondrial phospholipid) fully restored this enzyme activity, confirming cardiolipin's crucial role.

Area of Science:

  • Biochemistry
  • Mitochondrial Biology
  • Endocrinology

Background:

  • Cardiolipin is essential for mitochondrial membrane structure and function.
  • Previous studies indicated reduced cytochrome c oxidase activity in hypothyroid rat hearts.
  • A potential role for cardiolipin in this reduction was hypothesized.

Purpose of the Study:

  • To investigate the role of cardiolipin in thyroid hormone-induced changes in mitochondrial function.
  • To determine if exogenous cardiolipin can restore decreased cytochrome oxidase activity in hypothyroid mitochondria.

Main Methods:

  • Utilized the liposome-mitochondria fusion technique to increase cardiolipin content in mitochondria.
  • Assessed cytochrome c oxidase activity in heart mitochondria from hypothyroid and control rats.

Related Experiment Videos

  • Compared the effects of exogenous cardiolipin versus other phospholipids on enzyme activity.

    • Main Results:

    • The decreased cytochrome c oxidase activity in hypothyroid rat heart mitochondria was significantly restored by adding exogenous cardiolipin.
    • Other phospholipids did not restore the enzyme activity.
    • The restoration of activity by cardiolipin was complete, reaching levels comparable to control rats.

    Conclusions:

    • Cardiolipin plays a critical role in regulating thyroid hormone-dependent changes in mitochondrial cytochrome oxidase activity.
    • The data strongly support the involvement of cardiolipin in the functional alterations of mitochondria observed in hypothyroidism.