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Long-range interaction between the P2.1 and P9.1 peripheral domains of the Tetrahymena ribozyme
1Department of Chemistry, Faculty of Science, Kyoto University, Kyoto 606-01, Japan.
Nucleic Acids Research
|May 1, 1997
Abstract:
The Tetrahymena ribozyme possesses peripheral domains, termed P9.1 and P9.2. They are nonessential in the mechanism of the catalytic reaction but contribute to enhance the catalytic activity of the ribozyme. It has been postulated that P9.1 is capable of forming Watson-Crick base pairings with another peripheral domain, P2.1. We report here the existence of long-range base pairings between the loop regions of these two domains and show that this interaction apparently plays a role in enhancing the catalytic activity of the ribozyme.