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Related Experiment Videos

Cre-mediated somatic site-specific recombination in mice

K Akagi1, V Sandig, M Vooijs

  • 1Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

Nucleic Acids Research
|May 1, 1997
PubMed
Summary

Conditional mutant mice using Cre/lox systems are valuable for studying gene function and disease models. A new flox LacZ mouse strain effectively monitors Cre-mediated recombination across various tissues.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Conditional mutant mice with recombination systems (Cre/lox, Flp/frt) are crucial for tissue-specific gene function studies and disease modeling.
  • The effectiveness of these systems relies on cell target specificity, efficiency, and temporal control of gene activity.

Purpose of the Study:

  • To evaluate the utility of adenoviral vectors and transgenic mice expressing Cre for Cre/lox-mediated somatic recombination.
  • To assess the functionality of a newly generated flox LacZ reporter mouse strain for monitoring Cre activity.

Main Methods:

  • Administration of adenoviral Cre vectors via different routes in flox LacZ mice.
  • Generation and utilization of transgenic mice expressing Cre under tissue-specific promoters.

Related Experiment Videos

  • Monitoring LacZ reporter gene recombination and beta-galactosidase activity.

    • Main Results:

    • Adeno-Cre virus administration resulted in LacZ recombination and expression in numerous tissues.
    • Beta-galactosidase activity in the liver was transient due to cell turnover, while in other tissues it persisted for months.
    • The flox LacZ strain effectively reported Cre protein level and functionality.

    Conclusions:

    • The flox LacZ mouse line is a versatile tool for assessing Cre recombinase activity from various sources.
    • This model system aids in the study of gene function and the development of better disease models.