Related Experiment Videos
Matrix-assisted laser desorption/ionisation mass spectrometry of transfer ribonucleic acids isolated from yeast
I Gruic-Sovulj1, H C Lüdemann, F Hillenkamp
1Department of Biochemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia.
Nucleic Acids Research
|May 1, 1997
Abstract:
tRNATyr and tRNASer purified from bulk brewer's yeast tRNA were subjected to analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Choosing a mixture of 2,4,6- and 2,3,4-trihydroxy-acetophenone and diammonium citrate as matrix a mass resolution of up to 220 (FWHM) was achieved in the linear mode of operation. Cation adduct suppression by addition of cation exchange beads and a chelating agent (CDTA) is shown to substantially improve mass resolution for this class of molecules.