Adrenergic control of pineal N-acetyltransferase activity: developmental aspects

The activity of pineal N-acetyltransferase in the neonatal rat does not exhibit the large daily rhythm seen in the adult and is intermediate between the low day and high night adult values. These intermediate values appear to result from adrenergic stimulation. Blockade of adrenergic receptors or of catecholamine synthesis results in a decrease in enzyme activity in vivo. In vitro studies provide additional evidence of a completely developed postsynaptic adrenergic control system for pineal N-acetyltransferase activity at birth. Our observations indicate that the appearance of a circadian rhythm in pineal N-acetyltransferase at the end of the first week of life reflects the development of presynaptic mechanisms and structures necessary for the control of catecholamine release and uptake. These events follow the developmental appearance of the postsynaptic mechanisms required to mediate the adrenergic-cycle AMP regulation of pineal N-acetyltransferase activity, which can be detected prior to birth.