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Blood sample processing effect on eosinophil cationic protein concentration

N Rubira1, M J Rodrigo, M Pena

  • 1Department of Allergy, Hospital Universitario Vall d'Hebron, Universidad Autónoma de Barcelona, Spain.

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
|April 1, 1997
PubMed
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Sample processing significantly impacts eosinophil cationic protein (ECP) levels in asthma patients. Higher temperatures increase ECP, while EDTA prevents its release, crucial for monitoring treatment.

Area of Science:

  • Clinical immunology
  • Biochemistry

Background:

  • Asthma is an inflammatory disease where eosinophils and their mediators, like eosinophil cationic protein (ECP), are key indicators of severity.
  • ECP levels can be used to monitor asthma treatment efficacy.
  • Blood sample processing conditions are known to influence ECP measurements.

Purpose of the Study:

  • To investigate the impact of temperature, time, and anticoagulants on eosinophil cationic protein (ECP) levels.
  • To determine optimal conditions for ECP blood sample processing.

Main Methods:

  • Blood samples were collected from five asthmatic patients and five healthy controls.
  • Samples were processed with heparin, EDTA, or no anticoagulant.
  • Serum and plasma samples were subjected to varying temperatures (0°C, room temperature, 37°C) and storage times.

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  • ECP levels were quantified using fluoroimmunoassay (CAP-System ECP FEIA Pharmacia).

    • Main Results:

    • Elevated temperatures during blood clotting led to increased ECP concentrations.
    • ECP levels in serum and heparinized plasma were comparable, suggesting clotting may not be essential for in vitro ECP release.
    • EDTA plasma samples showed no ECP release at 0°C or room temperature.

    Conclusions:

    • Temperature and anticoagulant choice critically affect in vitro ECP measurements.
    • Further research is needed to fully elucidate the in vitro ECP release mechanism.