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Related Experiment Videos

Relationship of acrosin activity to sperm function tests

H Nakagawa1, H Okada, M Fujisawa

  • 1Department of Urology, Kobe University School of Medicine, Japan.

Andrologia
|March 1, 1997
PubMed
Summary

Lower sperm acrosin activity is linked to infertility. A combination of the Penetrak test and acrosin activity measurement can effectively predict results of the hamster egg-sperm penetration test for infertility diagnosis.

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Area of Science:

  • Reproductive Biology
  • Andrology
  • Infertility Research

Background:

  • Sperm function tests are crucial for diagnosing male infertility.
  • The zona-free hamster egg-sperm penetration test is a reliable indicator of sperm fertilizing capacity but is time-consuming and requires specialized equipment.
  • There is a need for alternative, clinically applicable sperm function tests.

Purpose of the Study:

  • To evaluate the diagnostic utility of acrosin activity, Penetrak test, and hypo-osmotic swelling test as alternatives to the hamster egg-sperm penetration test.
  • To determine if a combination of these tests can accurately predict the results of the hamster test.
  • To identify sperm function tests suitable for routine clinical use in evaluating male infertility.

Main Methods:

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  • Sperm samples from infertile patients and fertile volunteers were analyzed.
  • Acrosin activity was measured in spermatozoa.
  • The Penetrak test and hypo-osmotic swelling test were performed.
  • Results were compared with those of the zona-free hamster egg-sperm penetration test.

    • Main Results:

    • Sperm acrosin activity was significantly lower in infertile patients (asthenozoospermia, unexplained infertility) compared to fertile volunteers.
    • A combination of the Penetrak test and acrosin activity measurement achieved 88.2% accuracy in predicting the outcome of the zona-free hamster egg-sperm penetration test.
    • The hypo-osmotic swelling test showed less predictive value compared to the combined acrosin and Penetrak tests.

    Conclusions:

    • Measurement of acrosin activity and the Penetrak test are valuable sperm function assays.
    • A combined approach using acrosin activity and the Penetrak test can serve as an effective and practical alternative to the hamster egg-sperm penetration test for infertility assessment.
    • The hamster test is recommended only when the Penetrak test or acrosin activity measurements yield abnormal results.