Changes in dorsal cochlear nucleus blood flow during noise exposure

Changes in dorsal cochlear nucleus (DCN) blood flow during noise exposure were assessed using both intravital microscopy (IVM) and laser Doppler flowmetry (LDF). Mature Syrian golden hamsters were anesthetized, tracheotomized and implanted with a carotid artery catheter for monitoring blood pressure and infusion of fluorescent dye. An occipital craniectomy was performed and the cerebellum partly aspirated for access to the DCN. Fluorescent dye was infused to enhance observations of the DCN surface using a customized IVM. Red blood cell velocity, vessel diameter and blood pressure were continuously monitored. Subjects were presented with 110 dB SPL broad-band noise for 15 min. A second group was presented with the same protocol for laser Doppler assessment of blood-flow changes. Control groups included animals not exposed to noise using both IVM and laser Doppler measures. Additional control measurements were obtained for noise-exposed and control groups with laser Doppler measures obtained from the obex, a brainstem structure with no known auditory function. Arterioles of the DCN showed a low-frequency oscillatory pattern of red blood cell velocity in control animals and in baseline conditions of the noise-exposure group. Presentation of noise abolished these velocity oscillations. Additionally, significant decreases in both red blood cell velocity and vessel diameter were measured during stimulation. These microvascular measures recovered slightly during the post-stimulus period. Laser Doppler measures of the overall blood flow in the nucleus were consistent with IVM findings. Measurements from DCN and obex in groups not exposed to noise, and animals exposed to noise and measured from the obex, showed stable oscillatory flow. These data show exposure to noise eliminates oscillatory patterns of blood flow and induces decreases in vascular perfusion. Furthermore, these changes appear specific to the auditory structure studied.