Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tympanic membrane changes in experimental purulent otitis media

M von Unge1, W F Decraemer, D Bagger-Sjöbäck

  • 1Department of Otorhinolaryngology, Karolinska Hospital, Stockholm, Sweden.

Hearing Research
|April 1, 1997
PubMed
Summary

This study reveals that middle ear infections reduce tympanic membrane (TM) stiffness, causing TM displacement and potential weak spots. These changes may lead to perforations and impact otitis media progression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reactions to Gudair® vaccination identified in sheep used for biomedical research.

Australian veterinary journal·2019
Same author

Three-dimensional vibration of the malleus and incus in the living gerbil.

Journal of the Association for Research in Otolaryngology : JARO·2014
Same author

Magnetically driven middle ear ossicles for optical measurement of vibrations in an ear with opened tympanic membrane.

The Review of scientific instruments·2014
Same author

Optoelectronic moire projector for real-time shape and deformation studies of the tympanic membrane.

Journal of biomedical optics·2012
Same author

Calculation of area change and volume displacement from moire topograms of deformed membranes.

Applied optics·2010
Same author

Automatic calibration method for phase shift shadow moire interferometry.

Applied optics·2010

Area of Science:

  • Otorhinolaryngology
  • Biomedical Engineering
  • Pathology

Background:

  • Otitis media is a common middle ear infection.
  • The tympanic membrane's mechanical properties are crucial for hearing.
  • Understanding TM changes during infection is vital for treatment.

Purpose of the Study:

  • To investigate the mechanical stiffness changes of the gerbil tympanic membrane (TM) during early purulent otitis media.
  • To correlate TM stiffness alterations with histological and physiological changes.

Main Methods:

  • Experimental otitis media induced by Streptococcus pneumoniae inoculation in gerbils (n=49).
  • In vitro tympanometry to measure acoustic admittance and susceptance.
  • Moire interferometry to assess TM displacement under static pressure.

Related Experiment Videos

  • Concurrent histological analysis of the TM.

    • Main Results:

    • Acoustic admittance and susceptance decreased in the early days post-inoculation.
    • TM stiffness reduced over time, indicated by increased pressure-induced displacement.
    • Histological analysis showed developing edema in the pars tensa.
    • Local deformation zones ('weak spots') appeared in the inferior pars tensa.

    Conclusions:

    • Early purulent otitis media significantly reduces gerbil tympanic membrane stiffness.
    • Edema and molecular changes likely contribute to TM stiffness loss.
    • TM 'weak spots' may predispose to retraction pockets and perforations, influencing otitis media progression.