Indobufen induced modification of phospholipid composition in diabetic platelets

The influence of indobufen (200 mg twice daily for 10 days) on platelet lipid peroxidation and phospholipid metabolism in diabetic patients was investigated. We have studied 18 patients with type I of diabetes mellitus. The duration of disease varied between 3 and 25 years (average 11 years). All patients showed symptoms of diabetic angiopathy. Investigations were carried out after restoring the carbohydrate balance. The following tests were performed before and after a 10 day treatment with indobufen: malondialdehyde, conjugated dienes and lipid hydroperoxides, phospholipids, fatty acids. We observed a significantly higher production of lipid peroxidation products in diabetic platelets compared to control platelets. After a 10 day treatment with indobufen the intensity of peroxidation lowered significantly. The phospholipid composition of diabetic platelets showed a significant increase in lysophosphoglycerides fraction compared to the control platelets. After the indobufen treatment a significant decrease in sphingomyelin content was observed. In other phospholipid fractions no significant differences were observed.