A software program based on robust linear regression methods for instrumental calibration in biochemical analysis

A microcomputer program written in GWBASIC for conventional least squares regression (LS) and robust linear regression methods (RRMs) such as single median (SM) and repeated median (RM) is described. The main advantages of RRMs is the ability to detect outliers which can cause an inaccurate estimation of the analyzed samples. It requires MS DOS PC version 3.3 or higher and at least 256 kbyte of RAM. The joint application of RRMs and residuals analysis by the studentized method (STRM) allows a more precise biochemical analysis.