Characterization of O-antigens from different strains of Pseudomonas syringae pv. tabaci

O-Antigens (lipopolysaccharides, LPS) were isolated by NaCl extraction from microbial biomass of Pseudomonas syringae pv. tabaci and purified by ultracentrifugation. Individual structural components of the LPS macromolecule (O-specific polysaccharide (O-PS), core oligosaccharide, and lipid A) were obtained and characterized. Fatty acids 3-OH-C10:0, C12:0, 2-OH-C12:0, 3-OH-C12:0, C16:1, C16:0, C18:1, and C18:0 were identified in the lipid A composition. Glucosamine, ethanolamine, and phosphoethanolamine were found in the hydrophilic part of the lipid A macromolecule in all strains tested. Lipid A preparations contained phosphorus and amino acids. Rhamnose, glucose, glucosamine, 2-keto-3-deoxyoctulosonic acid, heptose, alanine, and phosphorus were identified as the main core components. The strains differed in O-PS structure. We describe the O-chain of LPS in strain P-28. It contains repeating units of the following structure: [formula: see text] The O-PS structures of LPS from strains P-28 and 225 are identical, however, they differ substantially from that of strain 223. Both structures from strains 223 and 225 were reported previously. Antibodies to antigenic epitopes of O-PS, core, and lipid A were revealed in O-serum against the whole bacterial cells. Correlation of O-PS structure with the serological grouping of strains was observed.