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Related Experiment Videos

Cell cycle-dependent establishment of a late replication program

M K Raghuraman1, B J Brewer, W L Fangman

  • 1Department of Genetics, University of Washington, Box 35-7360, Seattle, WA 98195-7360, USA. raghu@u.washington.edu

Science (New York, N.Y.)
|May 2, 1997
PubMed
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In yeast, telomeres delay DNA replication origin activation until late S phase. A signal established in G1 phase, not the telomere itself, dictates this temporal program.

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Genetics

Background:

  • Eukaryotic DNA replication origins are activated temporally during S phase.
  • Telomeres can influence origin activation timing, delaying it to late S phase.
  • The mechanism and timing of this telomere-mediated delay are not fully understood.

Purpose of the Study:

  • To investigate the role of cis-acting elements, specifically telomeres, in the temporal regulation of DNA replication origin activation.
  • To determine when the signal for late origin activation is established.
  • To understand if this signal requires continuous telomere presence.

Main Methods:

  • Utilized site-specific recombination in Saccharomyces cerevisiae (yeast) to separate DNA replication origins from telomeres in vivo.

Related Experiment Videos

  • Monitored the activation timing of these separated origins during the cell cycle.

    • Main Results:

    • Demonstrated that the signal for late S phase origin activation is established between mitosis and the G1 phase START event.
    • Showed that this signal persists through the subsequent S phase even after the origin is separated from the telomere.
    • Indicated that telomeres act as a trigger for establishing the delay, but are not required for its maintenance.

    Conclusions:

    • The temporal program of DNA replication origin activation is established early in the G1 phase.
    • Telomeres contribute to setting this program but are not continuously required for maintaining late-firing origins.
    • Origin initiation competence and the establishment of the temporal program may be linked events.