The morphology of the demineralized layer in primed dentin

This study critically examined the surface and subsurface effects of selected dentin priming agents. Various dentin priming agents were used and specimens were prepared for SEM examination by conventional air drying or by a freeze fracture and critical point drying technique. The results showed that in specimens prepared by freeze fracture and critical point drying technique the demineralization of the dentin surface and subsurface by priming agents leaves a visible network of collagen fibrils. This technique allows better evaluation of the adaptation of bonding resins to the collagen network of the demineralized layer.