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Related Experiment Videos

Apoptosis in oral erythema multiforme

E Chrysomali1, F Lozada-Nur, N P Dekker

  • 1School of Dentistry, University of California, San Francisco, USA.

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
|February 1, 1997
PubMed
Summary

Oral erythema multiforme involves significant keratinocyte apoptosis, indicated by increased apoptotic cells and altered expression of apoptosis-regulating proteins like p53. This suggests programmed cell death contributes to the condition.

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Histopathology meets molecular pathology.

Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics·2001

Area of Science:

  • Oral pathology
  • Dermatology
  • Molecular biology

Background:

  • Erythema multiforme is an inflammatory condition affecting oral mucosa.
  • The precise mechanisms of keratinocyte cell death in oral erythema multiforme are not fully understood.

Purpose of the Study:

  • To investigate the role of apoptosis in keratinocyte cell death in oral erythema multiforme.
  • To test the hypothesis that programmed cell death contributes to the pathogenesis of this condition.

Main Methods:

  • Immunohistochemical analysis of oral erythema multiforme and control biopsy specimens.
  • Evaluation of apoptosis-regulating proteins: Bcl-2, Bcl-x, Bax, p53, Fas, and Fas-ligand.
  • Quantification of apoptotic keratinocytes using morphologic criteria and TUNEL assay.

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Main Results:

  • Significantly higher numbers of apoptotic keratinocytes were observed in oral erythema multiforme compared to controls.
  • Overexpression of p53 protein was noted in basal keratinocytes of erythema multiforme specimens.
  • Reduced Bcl-x expression and altered Bax staining were observed in some erythema multiforme cases.

Conclusions:

  • Apoptosis is a significant mechanism of keratinocyte cell death in oral erythema multiforme.
  • Altered expression of apoptosis-regulating proteins, including p53, is implicated in the apoptotic process.
  • While direct evidence was limited, the Fas/Fas-ligand system may still play a role in inducing apoptosis.