Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular dissection of tight junctions

S Tsukita1, M Furuse, M Itoh

  • 1Department of Cell Biology, Faculty of Medicine, Kyoto University, Japan.

Cell Structure and Function
|October 1, 1996
PubMed
Summary

Researchers identified occludin, a key integral membrane protein, exclusively at tight junctions (TJ) in epithelial and endothelial cells. This discovery clarifies the TJ's role in barrier function and cell polarization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of in ovo feeding of L-leucine on amino acids metabolism and heat-shock protein-70, and -90 mRNA expression in heat-exposed chicks.

Poultry science·2018
Same author

Intraoperative 5-aminolevulinic acid-induced photodynamic diagnosis of metastatic brain tumors with histopathological analysis.

World journal of surgical oncology·2017
Same author

Oral administration of a medium containing both D-aspartate-producing live bacteria and D-aspartate reduces rectal temperature in chicks.

British poultry science·2017
Same author

First results of the 2.45 GHz Oshima electron cyclotron resonance ion source.

The Review of scientific instruments·2016
Same author

Effect of axial magnetic field on a 2.45 GHz permanent magnet ECR ion source.

The Review of scientific instruments·2016
Same author

Changes in free amino acid concentrations in the blood, brain and muscle of heat-exposed chicks.

British poultry science·2014

Area of Science:

  • Cell biology
  • Membrane protein research
  • Epithelial and endothelial cell biology

Background:

  • Tight junctions (TJs) are crucial for cellular barriers and polarization in epithelial and endothelial cells.
  • The integral membrane protein component of TJs has long been sought but remained elusive.
  • Understanding TJ protein function is vital for comprehending tissue integrity and function.

Purpose of the Study:

  • To identify and characterize the integral membrane protein localized at tight junctions.
  • To investigate the structure and function of this newly identified TJ protein.
  • To provide an overview of recent studies on occludin.

Main Methods:

  • Utilized monoclonal antibodies (mAbs) for protein identification.
  • Performed immunolocalization studies to determine protein location.
  • Conducted structure-function analyses of the identified protein.

Main Results:

  • Identified an integral membrane protein, occludin, exclusively localized at tight junctions.
  • Confirmed occludin's presence in both epithelial and endothelial cells.
  • Established occludin as a key component of the tight junction.

Conclusions:

  • Occludin is the elusive integral membrane protein localized at tight junctions.
  • Occludin plays a significant role in the barrier function and polarization mediated by TJs.
  • Further research into occludin's structure and function will enhance understanding of TJ biology.

Related Experiment Videos