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Total radical-trapping antioxidant parameter in NIDDM patients
A Ceriello1, N Bortolotti, E Falleti
1Department of Medicine and Pathology, Clinical and Experimental, University of Udine, Italy.
Diabetes Care
|February 1, 1997
Summary
Diabetic patients have lower antioxidant defenses, indicated by decreased total radical-trapping antioxidant parameter (TRAP) levels. This suggests TRAP may be a reliable measure for assessing oxidative stress in diabetes.
Area of Science:
- Biochemistry
- Endocrinology
- Oxidative Stress Research
Background:
- The role of oxidative stress in diabetes remains debated due to limitations in current assessment tools.
- Total radical-trapping antioxidant parameter (TRAP) offers a method to evaluate plasma antioxidant capacity, measurable directly (TRAPm) or calculated (TRAPc).
Purpose of the Study:
- To investigate oxidative stress in non-insulin-dependent diabetes mellitus (NIDDM) by comparing TRAP levels and key antioxidant markers between patients and controls.
- To assess the reliability of TRAP measurements in reflecting overall antioxidant status in diabetic individuals.
Main Methods:
- Evaluated malondialdehyde (MDA), TRAPm, TRAPc, protein-bound SH groups, uric acid, vitamin E, and vitamin C in 40 NIDDM patients and 40 matched controls.
- Correlated TRAPm and TRAPc values in both groups.
Main Results:
- NIDDM patients exhibited significantly lower TRAPm and TRAPc compared to controls.
- Diabetic subjects showed lower protein-bound SH groups and uric acid, but higher MDA and vitamin E levels.
- A strong correlation was observed between TRAPm and TRAPc in both NIDDM patients (r=0.68) and controls (r=0.74).
Conclusions:
- Decreased TRAP levels in NIDDM patients suggest compromised antioxidant defenses.
- TRAP provides a more comprehensive estimation of serum antioxidant capacity than individual antioxidant measurements.
- Calculated TRAP (TRAPc) may serve as a reliable and accessible tool for routine oxidative stress assessment in diabetic patients.