Related Experiment Videos
Phenylguanine found in urine after benzene exposure
K H Norpoth1, G Müller, C Schell
1Institute of Hygiene and Occupational Medicine, University Medical Center, Essen, Germany. tha020@sp2.power.uni-essen.de
Abstract:
Comparative investigations with synthetic N7-phenylguanine were carried out to clarify whether this compound is eliminated via the urine of rats as a benzene-derived nucleic acid adduct. As sensitive methods for detecting trace amounts of the compound, gas chromatography-mass spectroscopy, high performance liquid chromatography, and two immunoassays (enzyme-linked immunosorbent assay and fluoroimmunoassay) with appropriate monoclonal antibodies were used. The results indicate the excretion of several benzene-related guanine adducts slightly different from N7-phenylguanine that may possibly be hydroxylated. These adducts differ also from (O6-, N2- and C8-phenylguanine, respectively.