Related Experiment Videos
UVB-induced mutagenesis in hairless lambda lacZ-transgenic mice
A F Frijhoff1, H Rebel, E J Mientjes
1TNO Nutrition and Food Research Institute, Department of Molecular Toxicology, Zeist, The Netherlands.
Environmental and Molecular Mutagenesis
|January 1, 1997
Summary
This study shows that ultraviolet B (UVB) radiation causes mutations in hairless transgenic mice. The UVB-induced mutation frequency increased with dose, and the mutations were primarily G:C to A:T transitions.
Area of Science:
- Genetics
- Molecular Biology
- Dermatology
Background:
- Ultraviolet B (UVB) radiation is a known mutagen.
- Understanding UVB-induced mutagenesis is crucial for assessing skin cancer risk.
- Transgenic mouse models provide valuable tools for studying genotoxicity in vivo.
Purpose of the Study:
- To investigate UVB-induced mutagenesis in a hairless transgenic mouse model.
- To determine the dose-response relationship of UVB exposure on mutation frequency.
- To characterize the mutation spectrum induced by UVB.
Main Methods:
- Hairless 40.6 transgenic mice (MutaMouse) carrying the lambda gt10lacZ shuttle vector were used.
- Mice were exposed to single or fractionated doses of UVB radiation.
- LacZ mutant frequencies (MF) were quantified in the epidermis 23 days post-exposure.
- DNA sequencing was performed to analyze the mutation spectrum.
Main Results:
- LacZ MF exhibited a linear increase with increasing UVB dose.
- The mutagenic effects of successive UVB irradiations were additive.
- The predominant mutation type was G:C --> A:T transitions at dipyrimidine sites.
- Spontaneous mutations included insertions, deletions, and G:C --> A:T transitions at CpG sites.
Conclusions:
- The hairless lambda lacZ-transgenic mouse is a suitable in vivo model for studying UVB-induced mutations.
- UVB exposure leads to a dose-dependent increase in mutations, primarily G:C --> A:T transitions.
- This model aids in understanding the genotoxic mechanisms of UV radiation.