Related Experiment Videos
Removal of virus through novel membrane filtration method
1Fukuoka Women's University, Japan.
Developments in Biological Standardization
|January 1, 1996
Summary
A novel virus removal filter, PLANOVA, offers predictable and reproducible virus removal through a sieving mechanism. Its unique multi-layer pore structure ensures high efficiency in drug manufacturing and potential for broader applications.
Area of Science:
- Biotechnology
- Materials Science
- Virology
Background:
- Virus contamination poses a significant risk in biopharmaceutical manufacturing.
- Effective virus removal technologies are crucial for ensuring product safety.
Purpose of the Study:
- To introduce and validate PLANOVA, a novel virus removal filter technology.
- To demonstrate the predictable and reproducible virus removal capabilities of PLANOVA.
Main Methods:
- Utilized a sieving mechanism based on pore size (10-75 nm) and virus characteristics (size, deformability).
- Investigated the unique multi-layer pore structure (capillary and void pores) of the PLANOVA membrane.
- Employed regenerated cellulose for its hydroscopic properties to ensure good protein recovery.
Main Results:
- Demonstrated predictable virus removal based on pore size and virus properties under specific filtration conditions.
- Achieved high virus removability due to the membrane's unique multi-layer pore structure.
- Reported good protein recovery rates attributed to the membrane material.
Conclusions:
- PLANOVA offers a validated, reproducible, and predictable method for virus removal.
- The technology is effective in drug manufacturing and shows potential for wider applications, especially when combined with virus inactivation.
- Established integrity tests for membrane producers and users ensure reliable performance.