[Kinetics of dissociation of cyanide peroxidase complex]

The kinetics of dissociation ofHRP--CN- complex after reduction of the enzyme with ditionite was studied. The process goes in two consequent stages with different rate constants. The first stage can be related to the removal of CN- from Fe2+ and formation of out-of-equilibrium state of high-spin enzyme. The second stage possibly represents conformational relaxation of this state of the enzyme molecule.