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Related Experiment Videos

Pentane measurement in ventilated infants using a commercially available system

J A Drury1, J A Nycyk, R W Cooke

  • 1Institute of Child Health, Mersey Regional Neonatal Unit, Liverpool Women's Hospital, UK.

Free Radical Biology & Medicine
|January 1, 1997
PubMed
Summary

This study validates a method for measuring breath pentane in preterm infants, overcoming challenges like isoprene coelution and contamination. Reliable measurement of lipid peroxidation markers is now possible in this vulnerable population.

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Area of Science:

  • Biomedical Engineering
  • Neonatology
  • Analytical Chemistry

Background:

  • Breath pentane is a marker for lipid peroxidation, but its measurement is challenging.
  • Previous methods faced issues like isoprene coelution, exogenous contamination, and oxygen influence.
  • Accurate measurement is crucial for assessing oxidative stress in vulnerable infants.

Purpose of the Study:

  • To evaluate a specific gas chromatography method for measuring breath pentane in ventilated preterm infants.
  • To address and overcome known technical difficulties in breath pentane analysis.
  • To establish a reliable method for assessing lipid peroxidation in neonates.

Main Methods:

  • Utilized Chrompack 9001 Gas Chromatograph with thermal desorption and cryofocussing.

Related Experiment Videos

  • Employed an Al2O3/KCl PLOT column with Flame Ionization Detector (FID).
  • Separated isoprene from n-pentane and used hydrocarbon-free air to prevent contamination.

    • Main Results:

    • Successfully separated isoprene from n-pentane.
    • Established optimal sample storage conditions (Tedlar bags: 48h, desorption tubes: 24h).
    • Found no correlation between fractional inspired oxygen concentration (FiO2) and exhaled pentane levels.

    Conclusions:

    • The described method reliably measures breath pentane in ventilated preterm infants.
    • Careful methodology overcomes technical challenges and allows for accurate lipid peroxidation assessment.
    • This technique provides a valuable tool for monitoring infant health and treatment efficacy.