[Applicability of quantitative kinetic spectrophotometric method for glucose determination]

A simple kinetic method for quantitative determination of glucose has been developed. The method is based on two enzyme reactions, catalyzed by glucose oxidase (E. C. 1.1.3.4) and peroxidase (E. C. 1.11.1.7). The advantages of the method are high sensitivity (up to 2 X 10(-5) M glucose in a sample) and rapidity (3-4 min per analysis). The method gives reliable results for determination of small glucose concentrations in the presence of cellobiose. The conditions have been found when the reproducibility of the method does not depend on the glucose oxidase activity.