[Proteolytic activity of lysosomes from dog kidney under the effect of amphotericin B in vitro]

Amphotericin B at concentration of 0.1-1 mcg/ml is found to increase the yield of proteases from isolated dog kidney lysosomes. Further increase of amphotericin B concentration results in the decrease of proteolytic activity in the incubation medium, which is due to a considerable inactivation of proteases by high concentrations of antibiotic. Like amphotericin B, sodium deoxycholate, which is a part of many drugs, sharply inhibits the activity of enzyme, but, in contrast to the antibiotic, it almost does not release proteases from lysosomes. It is suggested that early described degradation of proteins of plasmic membranes and chromatin in dog kidney cells under the injection of amphotericin B is a result of the damage of lysosome membranes by the antibiotic.