Related Experiment Videos
[A novel diagnostic method for allergy "LUCICA HRT"]
M Yamakoshi1, Y Fujii, H Nagai
1Diagnostic Division, Asahi Chemical Industry Co., LTD, Tagata-gun.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|February 1, 1997
Summary
A new glass microfiber-based histamine release test (HRT) accurately diagnoses allergies. This test, evaluating both inhalation and food allergies, shows high concordance and specificity, proving useful for confirming clinical allergies.
Area of Science:
- Immunology
- Allergy Diagnostics
Background:
- Basophil leukocyte activation is key to allergic responses.
- Existing allergy tests have limitations in accuracy and specificity.
Purpose of the Study:
- To introduce and evaluate a novel glass microfiber-based histamine release test (HRT).
- To compare the diagnostic performance of HRT against established methods like CAP-RAST and skin tests.
Main Methods:
- Development of a novel glass microfiber-based histamine release test (HRT).
- Simultaneous determination of 20 allergens (10 inhalation, 10 food) using whole blood.
- Clinical evaluation involving 158 inhalation allergy patients and 135 food allergy patients.
Main Results:
- HRT demonstrated high concordance (78% inhalation, 84% food) and specificity (63% inhalation, 92% food).
- HRT exhibited a lower false positive ratio (14%) compared to CAP-RAST (42%).
- While CAP-RAST showed higher sensitivity, HRT proved effective in confirming clinical allergy status post-treatment.
Conclusions:
- HRT is a valuable diagnostic tool for confirming clinical allergies.
- CAP-RAST is effective for screening potential etiological allergens.
- HRT offers a reliable method for allergy diagnosis, particularly for monitoring treatment efficacy.