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[Multisite evaluation of a colorimetric broth microdilution antifungal susceptibility testing]
1Department of Laboratory Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|February 1, 1997
Summary
A new colorimetric method for antifungal susceptibility testing shows reliable results for yeast infections. This easy-to-use test is a suitable alternative for clinical microbiology labs.
Area of Science:
- Clinical Microbiology
- Mycology
- Antimicrobial Susceptibility Testing
Background:
- Accurate antifungal susceptibility testing is crucial for treating yeast infections.
- Existing methods can be time-consuming or complex.
- A need exists for a reliable, user-friendly assay.
Purpose of the Study:
- To evaluate a novel colorimetric microdilution antifungal susceptibility test.
- To assess its performance across multiple laboratories using reference and clinical yeast strains.
- To determine minimum inhibitory concentration (MIC) limits for specific antifungal agents.
Main Methods:
- A colorimetric microdilution assay using sodium resazurin as a dye was developed.
- The test was validated across 17 laboratories using ATCC reference strains and quality control strains.
- Antifungal susceptibility was tested against amphotericin B, flucytosine, fluconazole, miconazole, and itraconazole.
Main Results:
- 91.6% of 1,521 MIC determinations fell within acceptable limits.
- MIC limits for miconazole and itraconazole against reference strains were established.
- Clinical isolates showed varied susceptibility, with *Candida albicans* generally susceptible to the tested agents.
Conclusions:
- The colorimetric microdilution method is reliable and easy to perform.
- It serves as a suitable alternative for determining yeast MICs in clinical settings.
- Further validation confirmed its utility in routine laboratory diagnostics.