Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A physiologic function for alkaline phosphatase: endotoxin detoxification

K Poelstra1, W W Bakker, P A Klok

  • 1Department of Pharmacokinetics and Drug Delivery, University of Groningen, The Netherlands.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|March 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From benchtop to clinic: a translational analysis of the immune response to submicron topography and its relevance to bone healing.

European cells & materials·2021
Same author

The antifibrotic potential of a sustained release formulation of a PDGFβ-receptor targeted rho kinase inhibitor.

Journal of controlled release : official journal of the Controlled Release Society·2019
Same author

Pharmacokinetics of a sustained release formulation of PDGFβ-receptor directed carrier proteins to target the fibrotic liver.

Journal of controlled release : official journal of the Controlled Release Society·2017
Same author

Polymeric microspheres for the sustained release of a protein-based drug carrier targeting the PDGFβ-receptor in the fibrotic kidney.

International journal of pharmaceutics·2017
Same author

Pharmacokinetics of Succinylated Serum Albumin in Wistar Rats and Cynomolgus Monkeys: Implications for Dosage Regimens in the Therapy of HIV Infection.

Drug delivery·2016
Same author

OS053. Trophoblast invasion in the mesometrial triangle of atp infused pregnant rats.

Pregnancy hypertension·2015
Same journal

Changes in Three-Dimensional Intrahepatic Biliary Structures in Patients With Hepatobiliary Diseases Visualized Using Tissue-Clearing Methods.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Transcription Factor Ets-1 Is a Central Regulator of Redox Balance and Liver Regeneration Through epidermal growth factor (EGF) and transforming growth factor-β (TGF-β) 1 Signaling.

Laboratory investigation; a journal of technical methods and pathology·2026
See all related articles

Alkaline phosphatase (AP) may protect against endotoxin, a bacterial product. This enzyme dephosphorylates endotoxin at physiological pH, reducing its toxicity and potential for lethal complications.

Area of Science:

  • Biochemistry
  • Enzymology
  • Microbiology

Background:

  • Alkaline phosphatase (AP) is a widely studied enzyme, often used as a liver function marker.
  • Its physiological relevance remains unclear due to an in vitro pH optimum (around 10.5) outside the physiological range.
  • This high pH optimum may stem from protein preparation artifacts, leading to negatively charged groups near the active site.

Purpose of the Study:

  • To investigate the hypothesis that endotoxin, a phosphorylated bacterial substance, acts as a natural substrate for AP.
  • To explore AP's endotoxin dephosphorylating activity at physiological pH.
  • To assess AP's protective role against endotoxin toxicity in vivo.

Main Methods:

  • In vitro assays using intestinal and renal tissue specimens to measure AP activity towards endotoxin at physiological pH.

Related Experiment Videos

  • In vivo experiments in rats involving intradermal injections to assess endotoxin toxicity after AP exposure.

    • Main Results:

    • AP demonstrated endotoxin dephosphorylating activity in vitro at pH levels closer to the physiological range.
    • In vivo studies showed a significant reduction in endotoxin-induced inflammatory reactions after AP treatment.
    • These findings suggest AP can mitigate endotoxin's harmful effects.

    Conclusions:

    • Alkaline phosphatase may function as a protective agent against endotoxin, a common product of Gram-negative bacteria.
    • This enzymatic activity could be crucial in preventing severe complications associated with bacterial infections.
    • The study re-evaluates the biological role of AP beyond its use as a diagnostic marker.