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Updated: Aug 8, 2026
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Gene manipulation and transfer of the plasminogen and coagulation system in mice
1Center for Transgene Technology and Gene Therapy, Vlaams Interuniversitair Instituut voor Biotechnologie, Leuven, Belgium.
Insights
Gene targeting and transfer technologies illuminate the roles of blood coagulation and fibrinolysis in cardiovascular diseases and other processes. These methods offer potential for novel therapeutic strategies.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Science
Background:
- The balance between blood coagulation and fibrinolysis is crucial for hemostasis.
- Dysregulation of these systems contributes to cardiovascular disorders like thrombosis and atherosclerosis.
- These systems also play roles in embryonic development, wound healing, cancer, and brain function.
Purpose of the Study:
- To review insights gained from gene targeting studies on proteinase systems.
- To discuss adenovirus-mediated gene transfer for studying fibrinolytic genes.
- To explore novel therapeutic strategies for restenosis and thrombosis.
Main Methods:
- Gene targeting to manipulate genetic balance of proteinase systems.
- Gene transfer, specifically adenovirus-mediated transfer of fibrinolytic genes.
- Review of existing literature and studies.
Main Results:
- Gene targeting has provided definitive insights into the biological roles of coagulation and fibrinolysis.
- Adenovirus-mediated gene transfer is a valuable tool for research and potential therapeutic development.
- Understanding these systems is key to addressing restenosis and thrombosis.
Conclusions:
- Gene targeting and transfer technologies are powerful tools for elucidating complex biological systems.
- These genetic manipulation techniques offer promising avenues for developing new treatments for cardiovascular diseases.
- Further research into fibrinolysis holds potential for innovative therapeutic strategies.
Abstract:
The blood coagulation and the fibrinolytic (or plasminogen/plasmin) systems determine the balance between the formation and dissolution of blood clots, but in addition contribute to the pathogenesis of various cardiovascular disorders such as thrombosis, atherosclerosis, and restenosis. Furthermore, they participate in a variety of other (patho)biological processes such as embryonic development, reproduction, wound healing, cancer, and brain function. Two recently developed technologies, gene targeting and gene transfer, that allow manipulation of the genetic balance of these proteinase systems in a controllable manner have allowed a more definitive elucidation of the biological role of these systems. This review summarizes the insights that have been obtained from the gene targeting studies and discusses the use of adenovirus-mediated transfer of fibrinolytic genes to study and possibly to develop novel strategies for the treatment of restenosis and thrombosis.
