Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Application of a computer program to exclude additional unexpected antibodies

J M Rondeel1, A H van Opdorp, R B Dinkelaar

  • 1Department of Clinical Chemistry and Haematology, Drechtsteden Hospital, Dordrecht, The Netherlands.

Transfusion
|March 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Occurrence and management of an aberrant free T4 in combination with a normal TSH.

The Netherlands journal of medicine·2018
Same author

Examination of peripheral blood films using automated microscopy; evaluation of Diffmaster Octavia and Cellavision DM96.

Journal of clinical pathology·2006
Same author

[Reports of transfusion incidents: experiences from the first year of hemovigilance in the region of the former ZWN (South West Netherlands) blood bank in Rotterdam].

Nederlands tijdschrift voor geneeskunde·2003
Same author

[Six-month old girl with an anaphylactic reaction to rice flour, a rare food allergy].

Nederlands tijdschrift voor geneeskunde·2001
Same author

Improved bacteriological surveillance of haemodialysis fluids: a comparison between Tryptic soy agar and Reasoner's 2A media.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·1999
Same author

Different strategies in the laboratory diagnosis of autoimmune disease: immunofluorescence, enzyme-linked immunosorbent assay or both?

Annals of clinical biochemistry·1999

A new computer program automates the creation of red blood cell (RBC) exclusion panels (EPs) to prevent transfusion reactions in patients with existing RBC antibodies. This tool efficiently identifies compatible blood, reducing manual effort and improving patient safety.

Area of Science:

  • Transfusion Medicine
  • Immunology
  • Medical Informatics

Background:

  • Patients with prior red blood cell (RBC) antibodies risk developing new ones after antigen exposure.
  • Pre-transfusion screening must exclude the presence of these antibodies using specific RBC testing criteria.
  • Manual selection of appropriate RBCs for exclusion panels is time-consuming and requires expertise.

Purpose of the Study:

  • To develop and evaluate a computer program for automated generation of RBC exclusion panels (EPs).
  • To assess the program's effectiveness in meeting established criteria for excluding additional RBC antibodies.
  • To determine the utility of commercially available RBC panels in this automated process.

Main Methods:

  • A computer program was designed to select a minimum number of RBCs from commercial panels to form EPs.

Related Experiment Videos

  • The program prioritizes RBCs from homozygous donors and uses heterozygotes when homozygotes are unavailable.
  • The program's performance was tested using a historical dataset of 488 documented antibodies.

    • Main Results:

    • Successful EPs, meeting strict criteria, were generated in 61% of cases using 3-4 phenotypes.
    • In 39% of cases, EPs could not fully exclude 1-2 additional antibodies or required heterozygote cells.
    • Commercial panels showed minor variations in efficiency; none could exclude all antibodies for anti-c, anti-e, or anti-M.

    Conclusions:

    • Computer-aided EP construction efficiently determines if strict transfusion criteria can be met.
    • This automated approach significantly reduces the number of RBC suspensions needed for antibody exclusion.
    • The effectiveness of different commercial RBC panels was comparable, with slight variations.