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Related Experiment Videos

Immune response to neonatal genetic immunization

Y Wang1, Z Xiang, S Pasquini

  • 1Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104, USA.

Virology
|February 17, 1997
PubMed
Summary

Neonatal mice receiving plasmid DNA for rabies virus glycoprotein developed a robust immune response, including antibodies and T helper cells. This indicates the neonatal immune system can effectively respond to genetic immunization, challenging tolerance induction theories.

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Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Neonatal immune systems are often prone to immunological tolerance.
  • Genetic immunization offers a novel approach to vaccination.
  • Rabies virus glycoprotein is a key target for rabies virus vaccines.

Purpose of the Study:

  • To investigate the efficacy of genetic immunization in neonatal mice.
  • To determine if neonatal mice can mount an immune response to rabies virus glycoprotein via plasmid vector delivery.
  • To assess the potential for overcoming neonatal tolerance induction.

Main Methods:

  • Neonatal mice were inoculated within 24 hours of birth with a plasmid vector expressing rabies virus glycoprotein.
  • Humoral (antibody) and cellular (T helper cell) immune responses were analyzed.

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  • Immune responses were compared to those observed in vaccinated adult mice.

    • Main Results:

    • Mice inoculated neonatally with the plasmid DNA developed antibodies against rabies virus glycoprotein.
    • T helper cell responses to rabies virus glycoprotein were also detected in neonatally immunized mice.
    • The immune response in neonatal mice was comparable to that observed in adult mice.

    Conclusions:

    • The neonatal immune system can effectively respond to rabies virus glycoprotein delivered via plasmid-based genetic immunization.
    • Genetic immunization can overcome the typical induction of immunological tolerance in the early neonatal period.
    • This study highlights the potential of early-life genetic immunization for vaccine development.