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Flow injection fluorimetric determination of ascorbic acid based on its photooxidation by thionine blue
T Pérez-Ruiz1, C Martínez-Lozano, V Tomás
1Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
The Analyst
|February 1, 1997
Abstract:
The photooxidation of ascorbic acid sensitized by Thionine Blue was studied. The Leucothionine Blue formed during the reaction is highly fluorescent. A flow injection method using merging zones is proposed for the determination of ascorbic acid over a concentration range from 8 x 10(-7) to 5 x 10(-5) mol l-1 with a throughout of 80 samples per h. The method was used for the simple and rapid determination of ascorbic acid in pharmaceuticals, fruit juices and soft drinks.