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High-performance liquid chromatographic determination of some amino acids after derivatization with
A M el-Brashy1, S M al-Ghannam
1Department of Chemistry, Girls College of Science, Dammam, Kingdom of Saudi Arabia.
The Analyst
|February 1, 1997
Abstract:
2-Hydroxy-1-naphthaldehyde (HNA) was investigated as a derivatizing reagent for the fluorescence detection of histidine, methionine and tryptophan at 385 and 265 nm. The separation of HNA-derivatized amino acids on a conventional reversed-phase column was achieved in less than 40 min. This method was compared with the reported o-phthaldialdehyde (OPA) method. The procedure was successfully applied to the assay of the pure amino acids in a mixture and their pharmaceutical dosage forms.