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Inexpensive 4-hour micro-agar dilution susceptibility determination method

Y Holloway1, J A Snijder, J Schiphuis

  • 1Research Laboratory, Public Health Laboratory, Groningen, The Netherlands.

Antimicrobial Agents and Chemotherapy
|December 1, 1996
PubMed
Summary

The micro-agar dilution (MAD) method provides rapid and inexpensive antibiotic susceptibility testing. This technique demonstrates high accuracy and reproducibility, making it suitable for routine and research laboratories.

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Area of Science:

  • Microbiology
  • Pharmacology
  • Clinical Diagnostics

Background:

  • Accurate antibiotic susceptibility testing is crucial for effective treatment.
  • Traditional methods can be time-consuming and costly.
  • A need exists for rapid, cost-effective antimicrobial susceptibility testing methods.

Purpose of the Study:

  • To evaluate the accuracy and reproducibility of the micro-agar dilution (MAD) method.
  • To compare the MAD method with traditional agar dilution techniques.
  • To assess the cost-effectiveness of the MAD method for antibiotic susceptibility testing.

Main Methods:

  • 18 antibiotics were tested against 29-32 microorganisms using the micro-agar dilution (MAD) method.
  • MICs were read microscopically after 4-h incubation and macroscopically after 18-h incubation.

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  • Results were compared to traditional agar dilution as the gold standard.

    • Main Results:

    • Microscopic MAD MICs showed 87.1% identical results and 97.9% within one dilution.
    • Macroscopic MAD MICs showed 86.8% identical results and 98.4% within one dilution.
    • Correlation with agar dilution was high (94.3% microscopic, 97.6% macroscopic).

    Conclusions:

    • The micro-agar dilution (MAD) method is a reproducible and accurate technique for antibiotic susceptibility testing.
    • The method is rapid, inexpensive, and suitable for routine and research laboratories.
    • High correlation with agar dilution supports its clinical utility.