Mutagenic effects of the Excimer laser using a fibroblast transformation assay

For over 10 years fiber-guided laser systems have been used in arthroscopic surgery. The possibility of a mutagenic risk by the use of lasers has not been sufficiently repudiated, especially for the Excimer laser at a wavelength of 308 nm, which is close to the absorption spectrum of DNA. The aim of this study therefore was to approximate the mutagenic risk of UV-laser wavelengths using an in vitro transformation assay. BALB/3T3 fibroblasts were irradiated with Excimer laser wavelengths of 248 nm and 308 nm. The resulting transformation rates were compared to those of untreated cells and to cells that were subjected to an x-ray dose of 1 Gy. Pulse energy, frequency, and irradiation time were varied over a wide range, from sublethal fluences to total cell death. Furthermore, the effect of repeated irradiation (split-dose) was analyzed. The results for the 248 nm irradiation showed a highly significant transformation rate (P < 10(-9)). In contrast, results for the 308 nm irradiation were not at all significant (P < .18). The transformation rate of cells that were treated with x-rays was increased exponentially compared with the controls. Although an increased transformation rate was found after 248 nm irradiation, that of the wavelength 308 nm was not enhanced. The study therefore shows that the use of the Excimer wavelength 308 nm in arthroscopic procedures does not imply a specific mutagenic risk.