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Related Experiment Videos

A solid-phase enzyme immunoassay for serum ferritin

L Thériault, M Pagé

    Clinical Chemistry
    |November 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new enzyme immunoassay for measuring human serum ferritin. The assay uses stable reagents and accurately quantifies ferritin levels, with a lowest detectable concentration of 5 microgram/liter.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Clinical Chemistry

    Background:

    • Ferritin is a key protein for iron storage in the body.
    • Accurate measurement of serum ferritin is crucial for diagnosing various conditions, including iron deficiency anemia and overload disorders.

    Purpose of the Study:

    • To develop and validate a novel enzyme immunoassay (EIA) for quantifying human serum ferritin.
    • To establish a sensitive and stable method for routine clinical laboratory use.

    Main Methods:

    • An antibody-based enzyme immunoassay was developed using polystyrene tubes.
    • Antibodies against human ferritin were adsorbed onto tubes, followed by reaction with serum ferritin and an alkaline phosphatase-labeled anti-ferritin antibody.
    • The amount of bound enzyme conjugate was measured to determine ferritin concentration.

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    Main Results:

    • The developed enzyme immunoassay demonstrated a direct proportionality between bound enzyme conjugate and serum ferritin levels.
    • Reagents proved to be stable, maintaining efficacy for extended periods when stored at 4 degrees C.
    • Average serum ferritin levels in healthy adults were determined: 58 microgram/liter for men and 43 microgram/liter for women.
    • The assay achieved a low limit of detection, down to 5 microgram/liter.

    Conclusions:

    • The described enzyme immunoassay provides a stable and sensitive method for human serum ferritin determination.
    • This assay is suitable for routine clinical diagnostics, offering reliable quantification of ferritin levels.