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Related Experiment Videos

Monocytes from mobilized stem cells inhibit T cell function

K Ino1, R K Singh, J E Talmadge

  • 1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660, USA.

Journal of Leukocyte Biology
|May 1, 1997
PubMed
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Peripheral blood stem cell products contain CD14+ monocytes that induce T cell apoptosis. These monocytes inhibit T cell function through cell contact, potentially involving cytokines like IL-10.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Mobilized peripheral blood stem cell (PSC) products and post-transplant leukocytes can suppress immune responses.
  • Understanding the cellular mechanisms behind this immune suppression is crucial for improving transplant outcomes.

Purpose of the Study:

  • To identify and characterize the cells within PSC products responsible for T cell apoptosis.
  • To elucidate the mechanisms by which these cells inhibit T cell function.

Main Methods:

  • Percoll density fractionation to isolate low-density cells.
  • Immunophenotyping using CD14, CD4, CD8, and TCR markers.
  • Assessing T cell mitogenic response using phytohemagglutinin (PHA).
  • Investigating cell-cell contact requirements for T cell inhibition.

Related Experiment Videos

  • Analyzing DNA fragmentation via Southern and TUNEL assays.
  • Gene expression analysis using reverse-transcriptase polymerase chain reaction (RT-PCR).

    • Main Results:

    • Low-density CD14+ monocytes were identified as the primary cells inducing T cell apoptosis in PSC products.
    • Purified CD4+ and CD8+ T cells showed normal mitogenic responses, indicating monocytes are responsible for inhibition.
    • T cell inhibition by CD14+ monocytes required direct cell-cell contact.
    • Evidence of activation-induced T cell apoptosis was observed in the presence of CD14+ monocytes.
    • High levels of interleukin-10 (IL-10) and tumor necrosis factor (TNF) gene transcripts were detected, suggesting their potential role in T cell inhibition.

    Conclusions:

    • CD14+ monocytes in mobilized peripheral blood stem cell products actively induce T cell apoptosis and suppress T cell function.
    • This inhibition is mediated by cell-cell contact and potentially influenced by cytokine production (IL-10, TNF).
    • These findings highlight a key mechanism of immune modulation in stem cell transplantation.