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Complete DNA sequence of canine adenovirus type 1
M D Morrison1, D E Onions, L Nicolson
1Department of Veterinary Pathology, University of Glasgow Veterinary School, UK. gvpa15@udcf.gla.ac.uk
The Journal of General Virology
|April 1, 1997
Summary
Researchers sequenced the canine adenovirus type 1 genome, identifying 30 open reading frames (ORFs). This canine virus shares structural similarities with human adenoviruses, particularly in its late region genes.
Area of Science:
- Virology
- Genomics
- Molecular Biology
Background:
- Canine adenovirus type 1 (CAV-1) is a significant pathogen in dogs.
- Understanding the complete viral genome is crucial for developing diagnostics and therapeutics.
- Previous studies have characterized aspects of CAV-1, but a complete sequence was lacking.
Purpose of the Study:
- To determine the complete DNA sequence of a field strain of canine adenovirus type 1.
- To identify and characterize the open reading frames (ORFs) within the viral genome.
- To compare the genomic organization and sequence identity of CAV-1 with human adenoviruses.
Main Methods:
- Sequencing of random fragments of viral DNA cloned into the pBluescript vector.
- Bioinformatic analysis to assemble the complete genome and identify ORFs.
- Comparative sequence analysis against known adenovirus genomes.
Main Results:
- The complete genome of canine adenovirus type 1 was determined to be 30,536 base pairs (bp).
- The genome is flanked by identical 161 bp inverted terminal repeats.
- Thirty ORFs were identified, showing arrangement into discrete regions similar to human adenoviruses; however, E3 and E4 ORFs exhibited minimal sequence identity with human counterparts.
Conclusions:
- The complete DNA sequence provides a foundational resource for canine adenovirus type 1 research.
- Genomic comparisons reveal both conserved and divergent features between canine and human adenoviruses.
- The identified ORFs and their regional organization offer insights into viral gene expression and evolution.