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Eosinophil adhesion to human bronchial epithelial cells: regulation by cytokines
M Sato1, H Takizawa, T Kohyama
1Department of Internal Medicine and Physical Therapy, University of Tokyo School of Medicine, Bunkyo-ku, Japan.
Background:
Eosinophil infiltration into the airways and interaction with bronchial epithelial cells are important in the pathogenesis of asthma. The purpose of the present study was to elucidate the regulatory mechanisms of eosinophil adhesion to human bronchial epithelial cells.
Methods:
We cultured a human bronchial epithelial cell line, BEAS-2B, on a collagen-coated glass slide. Highly purified human eosinophils were added to each well to allow attachment to epithelium for 30 min. The number of attached eosinophils was counted.
Results:
Eosinophil adhesion to epithelial cells was increased when the BEAS-2B cells were pretreated with interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha). Although IFN-gamma upregulated ICAM-1 expression as shown by flow cytometry, specific neutralizing antibody to ICAM-1 failed to block eosinophil adhesion. Dexamethasone significantly suppressed eosinophil adhesion to bronchial epithelial cells.
Conclusion:
Eosinophil adhesion to bronchial epithelium was dynamically regulated by cytokines, and this process might be a target for therapeutic intervention in the treatment of asthma.