Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet-activating factor receptor. Gene structure and tissue-specific regulation

T Shimizu1, H Mutoh, S Kato

  • 1Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.

Advances in Experimental Medicine and Biology
|January 1, 1996
PubMed
Summary

The human platelet-activating factor receptor gene has two transcripts with different expression patterns. Transcript 1 is widely expressed and responds to inflammation, while Transcript 2 is tissue-specific and hormone-regulated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of DNA markers for discrimination between domestic and imported beef.

Meat science·2011
Same author

Genetically encoded fluorescent sensors of membrane potential.

Brain cell biology·2008
Same author

Histamine-2 receptor expression in gastric mucosa before and after Helicobacter pylori cure.

Alimentary pharmacology & therapeutics·2005
Same author

Helicobacter pylori eradication induces marked increase in H+/K+-adenosine triphosphatase expression without altering parietal cell number in human gastric mucosa.

Gut·2005
Same author

Pericryptal fibroblast sheath in intestinal metaplasia and gastric carcinoma.

Gut·2004
Same author

Cdx1 induced intestinal metaplasia in the transgenic mouse stomach: comparative study with Cdx2 transgenic mice.

Gut·2004

Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • The human platelet-activating factor receptor (PAFR) gene is located on chromosome 1.
  • Two distinct 5'-noncoding exons (Exon 1 and 2) possess unique transcription initiation sites and promoters.

Purpose of the Study:

  • To investigate the alternative splicing and differential expression of PAFR gene transcripts.
  • To identify regulatory elements and hormonal influences on PAFR gene expression.

Main Methods:

  • Northern blotting to analyze transcript expression in various tissues and cells.
  • In vivo and in vitro transcriptional assays, including CAT reporter assays and gel mobility shift assays.

Main Results:

  • PAFR Transcript 1 shows ubiquitous expression, particularly in eosinophilic cells and leukocytes, and responds to inflammatory stimuli.

Related Experiment Videos

  • PAFR Transcript 2 exhibits tissue-specific expression, absent in leukocytes and brain.
  • Hormonal treatments, including estrogen, retinoic acid (vitamin A), and thyroid hormone, differentially regulate PAFR expression, with specific hormone-responsive elements identified.

    • Conclusions:

    • Alternative splicing of the PAFR gene generates transcripts with distinct regulatory mechanisms and expression profiles.
    • Hormonal factors play a significant role in modulating PAFR expression, particularly for the tissue-specific transcript.
    • Understanding these regulatory pathways is crucial for comprehending PAFR's role in inflammatory and physiological processes.