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Related Experiment Videos

Octoxynol presence in bear-bile

G J Jhon1, J E Hong, H J Yoon

  • 1Department of Chemistry, Ewha Womans University, Seoul, Korea.

Biochemistry and Molecular Biology International
|April 1, 1996
PubMed
Summary

Octoxynol, a compound found in bear bile, was identified and its structure confirmed using advanced analytical techniques. This finding is crucial for understanding the chemical composition of bear bile and its potential applications.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Bear bile is a traditional medicine containing various bioactive compounds.
  • Octoxynol, a synthetic surfactant, has not been previously reported in natural sources like bear bile.
  • Understanding the chemical constituents of bear bile is essential for quality control and pharmacological research.

Purpose of the Study:

  • To investigate the presence and structure of octoxynol in dried bear bile.
  • To elucidate the co-extraction and micelle formation of octoxynol with bear bile glycolipids.
  • To confirm the chemical structure of the identified octoxynol using spectroscopic methods.

Main Methods:

  • Folch-Suzuki partition method for co-extraction of octoxynol and glycolipids.
  • DEAE-Sephadex A-25 column chromatography for glycolipid purification.

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  • High-Performance Liquid Chromatography (HPLC) for separation and detection.
  • Nuclear Magnetic Resonance (NMR) spectroscopy and Electrospray Tandem Mass Spectrometry (ESI MS/MS) for structural elucidation.

    • Main Results:

    • Octoxynol was successfully co-extracted with bear bile glycolipids, forming mixed-micelles.
    • HPLC analysis revealed five distinct peaks, with the fourth peak fraction identified as octoxynol.
    • NMR and ESI MS/MS analyses confirmed the structure of octoxynol as (CH(3))(3)C-CH(2)-C(CH(3))(2)-C(6)H(4)-(OCH(2)-CH(2))n-OH.

    Conclusions:

    • This study provides the first evidence of octoxynol presence in dried bear bile.
    • The findings highlight the importance of analytical chemistry in identifying unexpected compounds in natural products.
    • The confirmed structure of octoxynol from bear bile lays the groundwork for further research into its biological activity and origin.