Related Experiment Videos
Histamine and cyclic AMP in isolated canine parietal cells
The American Journal of Physiology
|November 1, 1979
Summary
Histamine stimulates cyclic AMP production in canine parietal cells, closely linking it to cellular responses. However, other stimulants like carbachol and gastrin do not share this direct cyclic AMP link.
Area of Science:
- Gastroenterology
- Cell Physiology
Background:
- Parietal cells are crucial for gastric acid secretion.
- Cyclic AMP (cAMP) is a key intracellular second messenger.
- The role of cAMP in mediating parietal cell responses to various stimuli is not fully elucidated.
Purpose of the Study:
- To investigate the relationship between cyclic AMP (cAMP) production and the functional responses of isolated canine parietal cells to histamine.
- To compare the effects of histamine with those of carbachol and gastrin on cAMP levels and cellular activity.
Main Methods:
- Isolated canine parietal cells were utilized.
- Measurements included cyclic AMP (cAMP) generation, oxygen consumption, and aminopyrine accumulation.
- The effects of histamine, metiamide, isobutylmethylxanthine (IMX), carbachol, and gastrin were assessed.
Main Results:
- Histamine significantly increased cAMP production, oxygen consumption, and aminopyrine accumulation.
- Metiamide dose-dependently inhibited histamine-stimulated cAMP generation and oxygen consumption.
- While histamine's effect on oxygen consumption plateaued at higher concentrations, cAMP generation continued to rise; carbachol and gastrin did not further increase cAMP levels beyond IMX alone.
Conclusions:
- A strong correlation exists between cAMP production and histamine's action on isolated canine parietal cells.
- The potentiating interactions between histamine, carbachol, and gastrin involve signaling pathways downstream of cAMP generation.