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Related Experiment Videos

IgG glycation and function during continuous ambulatory peritoneal dialysis

J C Davin1, A H Bouts, R T Krediet

  • 1Emma Children's Hospital, Amsterdam, The Netherlands.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|February 1, 1997
PubMed
Summary

Immunoglobulin G (IgG) glycation occurs during continuous ambulatory peritoneal dialysis (CAPD), potentially enhancing complement activation. This glycation in dialysate may impact anti-infection defenses in CAPD patients.

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Area of Science:

  • Immunology
  • Biochemistry
  • Nephrology

Background:

  • Immunoglobulin G (IgG) plays a role in anti-infection defenses during continuous ambulatory peritoneal dialysis (CAPD).
  • The Fc fragment's oligosaccharidic chains are vital for IgG effector functions.
  • The hypothesis tested is that IgG glycation occurs during CAPD, altering IgG properties.

Purpose of the Study:

  • To investigate IgG glycation during CAPD.
  • To assess the impact of glycation on IgG's interaction with complement and phagocytes.
  • To compare glycated IgG levels in CAPD patient sera and dialysates.

Main Methods:

  • Purified normal IgG was incubated with glucose solutions of varying concentrations and pH.
  • Glycated IgG was separated using affinity chromatography.

Related Experiment Videos

  • Complement activation (C3c deposition) and polymorphonuclear leucocyte (PMN) phagocytosis were assessed in vitro using Staphylococcus aureus Wood (STAW).
  • Glycated IgG percentages were compared between sera and dialysates from 12 CAPD patients.

    • Main Results:

    • In vitro IgG glycation was proportional to glucose concentration, incubation time, and pH.
    • Glycated IgG anti-STAW induced significantly higher C3c deposition compared to non-glycated IgG (0.96 vs 0.79; P = 0.027).
    • IgG glycation did not affect PMN phagocytosis.
    • Percentages of glycated IgG were higher in dialysates than in sera of CAPD patients (5.38% vs 4.56%; P = 0.006).

    Conclusions:

    • IgG glycation occurs in the peritoneal cavity during CAPD.
    • This glycation leads to enhanced complement activation.
    • This finding may explain elevated complement activation in CAPD dialysate and potentially compromises anti-infection mechanisms.