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Ochratoxin A in wheat: certification of two reference materials
G M Wood1, S Patel, A C Entwisle
1Leatherhead Food Research Association, Surrey, UK.
Food Additives and Contaminants
|April 1, 1997
Summary
Two certified reference materials for ochratoxin A in wheat were prepared and validated. These materials ensure accurate mycotoxin testing in food safety assessments.
Area of Science:
- Food Chemistry
- Analytical Chemistry
- Mycotoxicology
Background:
- Accurate determination of ochratoxin A (OA) in wheat is crucial for food safety.
- Previous intercomparisons established OA levels in wheat but required certified reference materials (CRMs).
Purpose of the Study:
- To prepare and certify two wheat reference materials for ochratoxin A content.
- To provide reliable standards for OA determination in wheat.
Main Methods:
- Preparation of a blank wheat CRM (CRM 471) and a naturally contaminated wheat CRM (CRM 472).
- Homogeneity and stability testing of the prepared materials.
- Certification of OA mass fraction by 15 laboratories using diverse analytical methods.
Main Results:
- CRM 471 certified OA content was < 0.6 microgram/kg.
- CRM 472 certified OA content was 8.2 micrograms/kg with an uncertainty of 1.0 microgram/kg.
Conclusions:
- Successfully prepared and certified two wheat reference materials for ochratoxin A.
- These CRMs provide essential tools for accurate mycotoxin analysis in wheat, supporting regulatory compliance and food safety.