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Updated: Jul 21, 2026

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MAME Models for 4D Live-cell Imaging of Tumor: Microenvironment Interactions that Impact Malignant Progression
Published on: February 17, 2012
On the stability of a tumour cell surface protease after exposure to 6N HCl
1Division of Biochemistry, School of Biological Sciences, University of Manchester, UK.
Anticancer Research
|March 1, 1997
Abstract:
Well defined acinar tumour cells in frozen sections were exposed to 6NHCl for 5 h at room temperature. A technique was designed to monitor the activity of the enzyme, guanidinobenzoatase (GB), on these tumour cells; this involved cross-linking the enzyme to the cell surface and challenging the active centre with known fluorescent probes which only bind to the functional enzyme. It was demonstrated that the enzymic activity can be regained by appropriate folding of the protein.

