A newly established mouse model of peritoneal dissemination in human pancreatic cancer

Tissue from a Suit-2 cell line derived from human pancreatic cancer was transplanted into the normal pancreas of Balb/c nu/nu mice using a physiological fibrin adhesive kit (Beriplast P). A minced tissue specimen (2-5 x 10(2) mg) was transplanted into normal pancreas in each nude mouse, and immediately thereafter 10 microliters of type A solution and 10 microliters of type Bsolution of Beriplast P were administered simultaneously at the transplanted area. The tumor tissue promptly adhered to the normal pancreas. Ten days after the implantation of cancer tissue, neovascularization had also already formed around the cancer tissue. The transplantation ratio of cancer tissue in normal pancreas was 100% (20/20). The capsule around the cancer tissue had not clearly formed. Twenty-one days after transplantation, the cancer cells were observed to be disseminated and closely fixed to the mesentery. The dissemination ratio of this cell line in the mouse mesentery was 85% (17/20) at 21 days after transplantation. This nude mouse model might thus be useful for investigating the development of human pancreatic cancer.